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B6;129-Cckbrtm1.1(cre)Mgmj/J

Cat. No.: CEMM-07251071

Common Name: Cckbr-cre
Cckbrcre knock-in mice have the endogenous cholecystokinin B receptor promoter/enhancer sequences directing expression of Cre recombinase. These mice are a Cre-lox tool allowing Cre recombination in Cckbr-expressing cells/tissues (including neurons of the ventromedial nucleus of the hypothalamus), and may be useful in studying feeding behavior, obesity and diabetes.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Cckbrtm1.1(cre)Mgmj
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Allele Name
targeted mutation 1.1
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Allele Attributes
Recombinase-expressing
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Gene Symbol
Cckbr
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Gene Name
cholecystokinin B receptor
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Chromosome
7
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MGI Accession ID
6834787
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Strain of Origin
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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Molecular Note
Using homologous recombination in ES cells, a T2A sequence and a CreER(T2) fusion gene were inserted immediately downstream of the cholecystokinin B receptor locus (Cckbr) on chromosome 7. The targeting vector contained, from 5' to 3', a viral 2A oligopeptide sequence (T2A; mediates ribosomal skipping), a Cre recombinase gene, a bovine growth hormone polyA sequence, an FRT site, a PGK-neo-polyA cassette, and an FRT site. Founder mice were bred with Flpo-expressing mice to delete the PGK-neo-polyA cassette.
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HUSBANDRY
Suggested Controls
C57BL/6J
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Breeding Considerations
Both heterozygous and homozygous mice are viable and fertile with normal breeding, and no reported gross phenotypic or behavioral abnormalities. When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice. Alternatively, homozygous mice may be bred together.
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Breeding Strategy
Heterozygote x Heterozygote
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For Research Use Only.
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