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B6N.129S1-Mrgprb4tm3(cre)And/J
Cat. No.: CEMM-07250436
The entire open reading frame of the Mrgprb4 (MAS-related GPR, member B4) gene was replaced by an mtdTomato-2A-NLSCre-Frt-PGK-Neomycin-FRT cassette in these knockout/knock-in allele mutant mice. When virally transduced, they are useful in studies of Mrgprb4-expressing sensory neurons. Constitutive expression of mtdTomato has been demonstrated in dorsal root ganglion (DRG) cells.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Mrgprb4tm3(cre)And
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| Allele Name |
targeted mutation 3
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| Allele Attributes |
Recombinase-expressing
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| Gene Symbol |
Mrgprb4
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| Gene Name |
MAS-related GPR
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| Chromosome |
7
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| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
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| MGI Accession ID | show more close |
| Site of Expression |
Constitutive Mrgprb4-specific expression of mtdTomato has been demonstrated in dorsal root ganglion cells.
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| Strain of Origin |
129S1/Sv-Oca2+ Tyr+ Kitl+
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| Molecular Note |
The entire open reading frame, encoded by a single exon, was replaced with an mtdTomato-2A-NLSCre-Frt-PGK-Neomycin-FRT cassette. The membrane-tagged tdTomato (mtdTomato) contains the 8 amino acids of the MARCKS sequence (MGCCFSKT) fused to the N-terminus of the full-length tdTomato and was connected to a nuclear localization signal (NLS)-tagged Cre recombinase via an intervening foot-and-mouth disease virus 2A peptide (FMDV 2A or F2A) sequence.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6NJ
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| Breeding Considerations |
Heterozygotes and homozygotes are viable and fertile.
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For Research Use Only.
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