Online Inquiry
B6N.Cg-Ccktm1.1(cre)Zjh/J
Cat. No.: CEMM-07250416
Common Name: Cck-IRES-Cre
The Cck-IRES-Cre knock-in allele has an internal ribosome entry site and Cre recombinase in the 3' UTR of the cholecystokinin locus (Cck). As such, Cre recombinase expression is directed to Cck-expressing cells (cholecystokinin positive neurons) by the endogenous promoter/enhancer elements of the cholecystokinin locus.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Ccktm1.1(cre)Zjh
show more
close
|
| Allele Name |
targeted mutation 1.1
show more
close
|
| Allele Attributes |
Recombinase-expressing
show more
close
|
| Gene Symbol |
Cck
show more
close
|
| Gene Name |
cholecystokinin
show more
close
|
| Chromosome |
9
show more
close
|
| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
show more
close
|
| MGI Accession ID | show more close |
| Site of Expression |
Cre recombinase activity is observed in cholecystokinin positive neurons (interneurons) of the cortex.
show more
close
|
| Strain of Origin |
(C57BL/6 x 129S4/SvJae)F1
show more
close
|
| Molecular Note |
A targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, a polyA sequence, and an FRT flanked neo cassette into the 3' untranslated region (after the translational termination site) of the cholecystokinin locus (Cck). This construct was electroporated into C57BL/6;129 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric males were bred with Actin-FLPe females (on a C57BL/6 congenic background) to achieve germline transmission and to remove the neo selection cassette. The resulting pups harboring the Cre targeted mutation were selected. These Cck-IRES-Cre mice were subsequently bred together for several generations (and the FLPe transgene was removed).
show more
close
|
| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6NJ
show more
close
|
| Breeding Considerations |
Mutant mice were bred to C57BL/6NJ inbred mice for several generations using a marker-assisted, speed congenic approach to establish this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.
show more
close
|
For Research Use Only.
Related Products