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STOCK Gt(ROSA)26Sortm6(Gli1)Amc/J
Cat. No.: CEMM-07250265
These RosaGli1Flag c/c mice contain a floxed-neomycin resistance (neo) cassette and polyadenylation signal, cDNA encoding a FLAG-tagged GLI-Kruppel family member (Gli1) gene, an internal ribosome entry site (IRES), and a Venus yellow fluorescent protein (YFP) under control of the ubiquitous Gt(ROSA)26Sor locus. These mice may be useful for understanding Sonic hedgehog signaling and identifying targets of Gli1 action in developing ventral neural tube.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm6(Gli1)Amc
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| Allele Name |
targeted mutation 6
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inserted expressed sequence
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
Gli1, GLI-Kruppel family member GLI1, mouse, laboratory; FLAG, FLAG octapeptide
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| MGI Accession ID | show more close |
| Site of Expression |
When mice carrying this allele are crossed to mice containing Cre-recombinase under direction of an atonal homolog 1 (Math1) promoter, active in dividing granule neuron precursor cells and medulloblastoma tumors, the mice produce Gli1Flag at levels higher than the endogenous protein in the cerebellum.
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| Strain of Origin |
129 and C57BL/6 and CBA and Swiss Webster
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| Molecular Note |
A full-length Gli1 cDNA followed by sequence for a C-terminal 3X FLAG tag, an IRES, the Venus fluorescent reporter gene, an intron and poly(A) signal sequences were inserted into the locus downstream of a splice acceptor and loxP site flanked neomycin resistance gene cassette. Upon Cre expression, the neo cassette is removed and FLAG tagged Gli1 gene and Venus gene expressed.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, homozygotes may be bred together.
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For Research Use Only.
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