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B6.FVB-Tg(CAG-EGFP, -ALPP)2.6Ggc/J
Cat. No.: CEMM-07250099
These piGAP transgenic reporter mice have expression of the eGFP-F-IRES-hPLAP dicistronic gene blocked by an unpstream loxP-flanked STOP-polyA sequence. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP-polyA sequence deleted in the cre-expressing tissue(s), permitting dicistronic expression of human Placental Alkaline Phosphatase (PLAP or ALPP) and farnesylated Enhanced Green Fluorescent Protein (eGFP-F; optimized to target expression to the cytoplasmic side of the plasma membrane). These piGAP transgenic reporter mice allow Cre-inducible, eGFP-F and hPLAP expression in multiple cell and tissue types.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Tg(CAG-EGFP,-ALPP)2.6Ggc
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| Allele Name |
transgene insertion 2.6
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Tg(CAG-EGFP,-ALPP)2.6Ggc
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| Gene Name |
transgene insertion 2.6
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| Chromosome |
UN
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| Expressed Genes |
ALPP, alkaline phosphatase, placental, human; GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
GFP and alkaline phosphatase are expressed following Cre-mediated excision of a transcriptional stop sequence.
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| Strain of Origin |
FVB/N
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| Molecular Note |
The transgene was designed with a CMV enhancer/chicken beta-actin promoter (from pCAGGS vector) and loxP-flanked STOP-polyA sequence upstream of a farnesylated Enhanced Green Fluorescent Protein coding sequence (eGFP-F; from pEGFP-F plasmid (Clontech), eGFP fused to the carboxyl-terminal 20 amino acids of the human c-Ha-Ras protein, which targets GFP to the cytoplasmic side of the plasma membrane), internal ribosomal entry site (IRES), human Placental Alkaline Phosphatase cDNA sequence (PLAP or ALPP; from the pGTO plasmid), and SV40 polyA signal.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J Noncarrier
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| Breeding Considerations |
Mutant mice were bred to C57BL/6J mice to generate this congenic strain. When maintaining the live congenic colony, hemizygous mice may be bred to wildtype siblings or to C57BL/6J inbred mice. The donating investigator reports that attempts to generate homozygous mice (on the FVB/NCrl genetic background) were not successful and suggests that homozygosity may be lethal at some embryonic stage.
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For Research Use Only.
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