B6;FVB-Ifi208^{Tg(Cspg4-cre)1Akik}/J

Ifi208Tg(Cspg4-cre)1Akik

transgene insertion 1

Recombinase-expressing

Ifi208

interferon activated gene 208

1

Cre, Cre recombinase, bacteriophage P1

3778062

NG2 expressing glial cells and vasculature throughout the brain as well as in NG2-expressing cells in other tissues from late embryonic stages (~embryonic day 14) throughout adulthood

(C57BL/6 x SJL)F1

The transgene construct consisted of the 208 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-309G21, containing the entire NG2 (Cspg4) gene (and 60 kbp of 5' and 114 kbp of 3' flanking sequences), which was modified by inserting an nls-Cre coding sequence followed by a rabbit beta-globin polyA signal into the first exon of the NG2 gene. The NG2 translation initiation ATG was changed to AAG to prevent translation from the BAC NG2 gene and to allow translation from the first ATG in the Cre sequence. Cre expression is detected only in Cspg4-expressing cells at P1, P14 and P60 and not in Gfap +ve astrocytes or APC +ve oligodendrocytes. Activity is also detected in male germ cells. Line 1 inserted into an intron of the gene at 173692115 (Build GRCm38/mm10). Founder line 1 has a copy number of 1-3.

Noncarrier

When maintaining a live colony, transgenic females may be bred to wildtype (non-carrier) male siblings. The donating investigator reports that NG2creBAC transgene expression may be more stable when passed through the female germline (i.e. transgenic females are bred with non-carrier males). Also, Cre recombinase may be expressed in male germ cells. Breeding through the male germline leads to copy number loss and poor expression.

Hemizygote x Noncarrier