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STOCK Igs6tm2.1(ACTB-EFGP, -tdTomato)Luo/J

Cat. No.: CEMM-07250452

Common Name: MADM-12GT (John12GT)
The MADM-12GT (Igs6GT) allele has the CMV enhancer/chicken beta-actin core promoter, the N-terminal portion of mut4-EGFP, a beta-globin intronic sequence (containing an frt site and loxP site), and the MYC-tagged C-terminal portion of tdTomato all inserted into the Igs6 locus on chromosome 12 (~1.71 cM; ~16 kbp downstream of exon 1 of the Rab10 gene). These MADM-12GT mutants are designed for MADM (mosaic analysis with double markers), and must be crossed to MADM-12TG mice harboring a reciprocal mutation at the same locus. This MADM system allows Cre recombinase-induced fluorescent labeling of daughter cells to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice may also be useful in studies of cell differentiation, mitosis, and imprinting.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Igs6tm2.1(ACTB-EFGP,-tdTomato)Luo
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Allele Name
targeted mutation 2.1
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Allele Attributes
Reporter; Null/Knockout
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Gene Symbol
Igs6
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Gene Name
intergenic site 6
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Chromosome
12
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Expressed Genes
RFP, Red Fluorescent Protein, coral; GFP, Green Fluorescent Protein
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MGI Accession ID
5491599
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Site of Expression
EGFP and tdTomato will be expressed in cells/tissues where promoters driving Cre recombinase and Flp recombinase are expressed.
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Strain of Origin
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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Molecular Note
A targeting vector was Created with a CMV enhancer/chicken beta-actin core promoter (pCA) upstream of the "GT MADM" cassette. The "GT MADM" cassette used here was designed with the N-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 1-273), a beta-globin intronic sequence containing an FRT site and loxP-flanked neomycin resistance gene, the C-terminal portion of a red fluorescent protein (tdTomato; aa 4-1544) tagged with three copies of the Myc epitope at itsC-terminus, and an SV40 T-antigen poly(A) signal. This entire "GT MADM" construct was inserted into the John12 locus on chromosome 12 (~1.71 cM; ~16 kbp downstream of exon 1 of the Rab10 gene). Cre-mediated recombination removed the floxed neo cassette.
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HUSBANDRY
Suggested Controls
B6129SF2/J C57BL/6J
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Breeding Considerations
When maintaining a live colony, homozygous mice may be bred together.
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For Research Use Only.
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