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B6.Cg-Snord116tm1Uta/J
Cat. No.: CEMM-07250095
Common Name: 2-loxp (floxed)
As deletions of the Snord116 cluster are associated with Prader-Willi syndrome (PWS), mice carrying the 2-loxP (floxed) allele may be useful in generating conditional mutations for studying the role of Snord116 in growth and feeding regulation, mechanisms of obesity, and pathophysiology of Prader-Willi syndrome.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Snord116tm1Uta
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); No functional change
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| Gene Symbol |
Snord116
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| Gene Name |
small nucleolar RNA
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| Chromosome |
7
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| MGI Accession ID | show more close |
| Strain of Origin |
B6.Cg-Thy1a
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| Molecular Note |
Two individual targeting vectors were used to place a loxP site (and an Frt1-flanked PGK-neo cassette) upstream, and a loxP site (and an Frt5-flanked puromycin resistance/TK cassette) downstream of the Snord116 cluster. The upstream targeting vector was transfected into C57BL/6-derived embryonic stem ES cells, and correctly targeted ES cells were next transfected with the downstream targeting vector. Doubly targeted ES cells were then transiently transfected with an FLP expressing plasmid to remove the two selection cassettes. The resulting 2-loxP ES cells (with a single loxP site just upstream, and a single loxP site just downstream of the Snord116 cluster.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred together. As imprinting of the endogenous gene is determined via paternal inheritance, paternal transmission of the mutant allele may be required.
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For Research Use Only.
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