B6.Cg-Tg(A930038C07Rik-cre)1Aibs/J

Cat. No.: CEMM-07250336

Common Name: A930038C07Rik-Tg1-Cre

These A930038C07Rik-Tg1-Cre mice express Cre recombinase under the control of the A930038C07Rik (RIKEN cDNA A930038C07 gene [also called epidermacan]) promoter/enhancer regions within the BAC transgene. These mice may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping in subgroups of brain tissues; specifically in neocortical deep layer 5 (Layer 5B) cells/thick-tufted pyramidal neurons of adult mice.

Inquiry

Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Tg(A930038C07Rik-cre)1Aibs show more close
Allele Name	transgene insertion 1 show more close
Allele Attributes	Recombinase-expressing show more close
Gene Symbol	Tg(A930038C07Rik-cre)1Aibs show more close
Gene Name	transgene insertion 1 show more close
Chromosome	UN show more close
Expressed Genes	Cre, Cre recombinase, bacteriophage P1 show more close
MGI Accession ID	5292251 show more close
Site of Expression	Cre recombinase activity/Cre expression (via in situ hybridization to Cre recombinase) in adult mice is observed as specific and unique to deep layer 5 (Layer 5B) cortical cells corresponding to thick-tufted pyramidal neurons. When crossing to a Cre-reporter line, reporter gene expression appears to be much more widespread (presumably due to more widespread Cre expression during development). These other regions include scattered cells in main olfactory bulb, anterior olfactory nucleus, taenia tecta, piriform areas, lateral septal nuclei, and inferior olivary nucleus. show more close
Strain of Origin	(C57BL/6 x C3H)F1 x C57BL/6 show more close
Molecular Note	The 196 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-476E22, containing the entire A930038C07Rik locus (and other genes), was modified by targeting a Cre recombinase coding sequence, SV40 polyA signal, and FRT flanked neomycin cassette to the ATG start site of the A930038C07Rik locus. The targeted BAC sequences were further modified using FLP recombination to remove the selection cassette and linearized to remove its original vector backbone. The resulting modified BAC was microinjected into embryos from B6C3 mice ((C57BL/6 x C3H)F1 X C57BL/6). Mice from founder line A930038C07Rik-Tg1-Cre were bred to C57BL/6J mice to generate the colony. Detection of Cre mRNA by in situ hybridization in adult mice is reported to be specific to layer 5 (Layer 5B) cortical cells corresponding to thick-tufted pyramidal neurons. Reporter activity in crosses to Cre reporter mice is more widespread, presumably due to Cre activity during development. This line demonstrates a much more specific expression pattern compared to mice from founder line 4 (Tg(A930038C07Rik-Cre)1Aibs). show more close

HUSBANDRY
Suggested Controls	C57BL/6J Noncarrier show more close
Breeding Considerations	When maintaining a live colony, hemizygous mice may be bred together, to wildtype (non-carrier) siblings, or to C57BL/6J inbred mice. The phenotype of homozygous mice has not yet been characterized. show more close

For Research Use Only.