Online Inquiry
B6.FVB-Agtr1aem1(cre)Zak/J
Cat. No.: CEMM-07250871
Common Name: Agtr1a-2A-Cre
Agtr1a-2A-Cre knock-in mice are designed to have Cre recombinase expression directed to Agtr1a-expressing cells. Cre activity recapitulates that of the endogenous Agtr1a gene in the brain. These mice may be used to generate conditional mutations for studying neural circuits underlying thirst, vasopressin secretion, and fluid homeostasis.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Agtr1aem1(cre)Zak
show more
close
|
| Allele Name |
endonuclease-mediated mutation 1
show more
close
|
| Allele Attributes |
Recombinase-expressing
show more
close
|
| Gene Symbol |
Agtr1a
show more
close
|
| Gene Name |
angiotensin II receptor
show more
close
|
| Chromosome |
13
show more
close
|
| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
show more
close
|
| MGI Accession ID | show more close |
| Site of Expression |
When these mice are bred with mice containing lox-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in Agtr1a-expressing cells in the offspring. _x000D_
Cre recombinase activity is detected in neurons of the forebrain lamina terminalis including the subfornical organ (SFO), organum vasculosum of the lamina terminalis (OVLT), and median preoptic nucleus (MnPO).
show more
close
|
| Strain of Origin |
FVB/N
show more
close
|
| Molecular Note |
The bicistronic Agtr1a-2A-Cre knock-in allele was Created using homologous recombination (aided by targeted CRISPR/Cas9 endonuclease activity) to insert a viral 2A oligopeptide sequence (T2A; mediates ribosomal skipping) and a Cre recombinase gene (Cre) immediately upstream of the endogenous STOP codon of Agtr1a gene. The targeting vector, Cas9 endonuclease and single guide RNA were mixed and injected into the pronuclei of FVB/N fertilized zygotes. Embryos were transferred to pseudopregnant females, and correctly targeted pups were maintained on mixed FVB/C57BL/6J background. Two independent knock-in lines were crossed to reporter mice, and reporter expression patterns from these lines were identical.
show more
close
|
| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
show more
close
|
| Breeding Considerations |
When maintaining a live colony, these mice can be bred as homozygotes.
show more
close
|
| Breeding Strategy |
Wild-type x Heterozygote; Heterozygote x Wild-type
show more
close
|
For Research Use Only.
Related Products