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FVB/N-Tg(CAG-EGFP, -ALPP)2.6Ggc/J

Cat. No.: CEMM-07250098

These piGAP transgenic reporter mice have expression of the eGFP-F-IRES-hPLAP dicistronic gene blocked by an upstream loxP-flanked STOP-polyA sequence. When bred to mice that express Cre recombinase, the resulting offspring will have the STOP-polyA sequence deleted in the cre-expressing tissue(s), permitting dicistronic expression of human Placental Alkaline Phosphatase (PLAP or ALPP) and farnesylated Enhanced Green Fluorescent Protein (eGFP-F; optimized to target expression to the cytoplasmic side of the plasma membrane). These piGAP transgenic reporter mice allow Cre-inducible, eGFP-F and hPLAP expression in multiple cell and tissue types.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Tg(CAG-EGFP,-ALPP)2.6Ggc
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Allele Name
transgene insertion 2.6
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Allele Attributes
Conditional ready (e.g. floxed); Reporter
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Gene Symbol
Tg(CAG-EGFP,-ALPP)2.6Ggc
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Gene Name
transgene insertion 2.6
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Chromosome
UN
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Expressed Genes
ALPP, alkaline phosphatase, placental, human; GFP, Green Fluorescent Protein
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MGI Accession ID
3797263
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Site of Expression
GFP and alkaline phosphatase are expressed following Cre-mediated excision of a transcriptional stop sequence.
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Strain of Origin
FVB/N
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Molecular Note
The transgene was designed with a CMV enhancer/chicken beta-actin promoter (from pCAGGS vector) and loxP-flanked STOP-polyA sequence upstream of a farnesylated Enhanced Green Fluorescent Protein coding sequence (eGFP-F; from pEGFP-F plasmid (Clontech), eGFP fused to the carboxyl-terminal 20 amino acids of the human c-Ha-Ras protein, which targets GFP to the cytoplasmic side of the plasma membrane), internal ribosomal entry site (IRES), human Placental Alkaline Phosphatase cDNA sequence (PLAP or ALPP; from the pGTO plasmid), and SV40 polyA signal.
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HUSBANDRY
Suggested Controls
FVB/NJ Noncarrier
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Breeding Considerations
When maintaining a live colony, hemizygous mice may be bred to wildtype siblings or to FVB/NJ inbred mice. The donating investigator reports that attempts to generate homozygous mice were not successful and suggests that homozygosity may be lethal at some embryonic stage.
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For Research Use Only.
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