B6(D2)-Tg(CAG-Brainbow1.0)2Eggn/J

Tg(CAG-Brainbow1.0)2Eggn

transgene insertion 2

Reporter

Tg(CAG-Brainbow1.0)2Eggn

transgene insertion 2

UN

RFP, Red Fluorescent Protein, coral; GFP, Green Fluorescent Protein; YFP, Yellow Fluorescent Protein, jellyfish

5464115

Expression is reportedly seen in E3.5, 4.5, 6.5, and 7.5 embryos as well as neonates. In adult mice, expression has been observed in skeletal and cardiac muscle, kidneys, lungs, brain, spinal cord, and a portion of the eye, stomach, pancreas, and intestine. By FACS, expression has been observed in thymocytes and splenocytes, as well as CD4, CD8, CD19, CD11b and CD11c positive cells.

(C57BL/6 x DBA/2)F1

The allele has a CAG hybrid promoter,a rox-flanked His3-SV40 transcriptional STOP cassette, an FRT flanked STOP cassette and a variant lox-flanked region containing an eGFP cassette, an inverted hM3Dq/mCherry fusion protein, WPRE, polyA signal and an attB/attP-flanked PGK-FRT Neo-polyA cassette all inserted into the Gt(ROSA)26Sor locus. The inverted hM3Dq-mCherry-2ACT88 fusion protein cDNA sequence contains (hM3Dq DREADD) a red fluorescent protein mCherry and the final 264 coding nucleotides of the rat Htr2A gene (encoding a C-terminal epitope that mediates localization to the cell body/soma and dendrites). PhiC31-mediated recombination removed the AttB/AttP-flanked neo sequence. Dre-mediated recombination removed the rox-flanked STOP cassette. Flp-mediated recombination removed FRT flanked STOP cassette.

C57BL/6J Noncarrier

Hemizygotes are viable and fertile. Homozygous mice show an elevated tendency for seizures and early death not seen in the hemizygotes, likely due to high expression of dTomato.