STOCK Tg(CAG-cre/Esr1*)5Amc/J

Tg(CAG-cre/Esr1*)5Amc

transgene insertion 5

Recombinase-expressing; Inducible

Tg(CAG-cre/Esr1*)5Amc

transgene insertion 5

UN

cre/Esr1, Cre recombinase and estrogen receptor 1 fusion gene

2182767

Tamoxifen-inducible cre; widespread pattern of expression; Tamoxifen administration will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice

(C57BL/6 x CBA)F1

This transgene expresses a fusion protein consisting of Cre recombinase joined to the ligand-binding domain of a mouse estrogen receptor modified to bind to 4-hydroxytamoxifen, but not to endogenous estrogen. The CAG promoter, containing a chicken beta actin promoter/enhancer coupled with the cytomegalovirus immediate-early (CMV-IE) enhancer, drives high levels of expression in most tissues. In the presence of tamoxifen, the fusion protein is transported into the nucleus, where Cre can excise loxP-flanked segments from conditionally modified genes.

Noncarrier

Homozygous mice are not viable or fertile. Transgene carrier mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When maintaining our live colony, carrier mice may be bred with wildtype (non-carrier) mice from the colony.

Heterozygote x +/+ sibling