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B6.Cg-Pabpn1tm1.1Gpvl/J
Cat. No.: CEMM-07250716
Common Name: Pabpn1 conditional knock-in
Pabpn1 conditional knock-in mice possess a 17-alanine (17) expanded Pabpn1 gene. Expression of this knock-in is prevented by an upstream floxed sequence containing a WT mouse Pabpn1 gene and an EGFP sequence. Cre Recombinase excision of the floxed region results in the expression of Pabpn1A17, associated with the onset of Oculopharyngeal muscular dystrophy (OPMD).
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Pabpn1tm1.1Gpvl
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| Allele Name |
targeted mutation 1.1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inserted expressed sequence
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| Gene Symbol |
Pabpn1
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| Gene Name |
poly(A) binding protein
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| Chromosome |
14
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| Expressed Genes |
GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have the floxed sequence removed, allowing for expression of Pabpn1A17 in Cre-expressing tissues.
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| Strain of Origin |
B6.Cg-Thy1a
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| Molecular Note |
The gene was targeted with a construct containing the following elements: a loxP site, the wildtype cDNA, an internal ribosomal entry site (IRES), an enhanced green fluorescent protein (eGFP) reporter gene, an FRT site flanked neomycin resistance gene, a second loxP site, and the portion of the human cDNA representing a (GCG)7 alanine codon expansion. This was targeted to immediately downstream of the ATG start codon in exon 1 and adds 7 alanines to the endogenous 10. Subsequent Flp-mediated recombination removed the neo cassette. This alanine expansion allele mimics a mutation found in oculopharyngeal muscular dystrophy (OPMD) patients.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, mice heterozygous for the floxed allele may be bred to wildtype littermates from the colony, or to C57BL/6J inbred mice. The donating investigator has not attempted to make this strain homozygous.
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| Breeding Strategy |
Wild-type x Heterozygote; Heterozygote x Wild-type
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For Research Use Only.
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