C57BL/6-Tg(ACTB-MAP2K1*K97M)1Stl/J

Cat. No.: CEMM-07250057

Common Name: dnMEK1

These mutant mice possess a Lox-STOP-Lox cassette controlling the inserted human dominant-negative mutant form of human MEK1. This strain may be useful for generating conditional mutations in studies involving synaptic plasticity and memory.

Inquiry

Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Tg(ACTB-MAP2K1*K97M)1Stl show more close
Allele Name	transgene insertion 1 show more close
Allele Attributes	Dominant negative; Inserted expressed sequence; Humanized sequence show more close
Gene Symbol	Tg(ACTB-MAP2K1*K97M)1Stl show more close
Gene Name	transgene insertion 1 show more close
Chromosome	UN show more close
Expressed Genes	MAP2K1, mitogen-activated protein kinase kinase 1, human show more close
MGI Accession ID	3707556 show more close
Site of Expression	Cre excision results in the removal of a stop codon, enabling kinase expression. show more close
Strain of Origin	C57BL/6 show more close
Molecular Note	The transgene encodes a dominant-negative mutant (K97M) form of human MEK1 (or MAP2K1). Expression under the control of the chicken Actin beta promoter occurs only following Cre-mediated removal of the upstream Lox-STOP-Lox cassette. In the absence of Cre recombinase, the transgene is not detectably expressed in the brains of these floxed mice. If bred to a Cre-expressing strain, the floxed stop region is excised in the resulting offspring, and mutant MEK1 expression is observed in the Cre-expressing tissue(s). show more close

HUSBANDRY
Suggested Controls	C57BL/6J Noncarrier show more close
Breeding Considerations	When maintaining a live colony, hemizygotes are bred to wildtype siblings or to C57BL/6J inbred mice. show more close

For Research Use Only.