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STOCK Gt(ROSA)26Sortm1(tTA)Roos/J

Cat. No.: CEMM-07250119

These ROSA:LNL:tTA mutant mice harbor a conditional mutation of the Gt(ROSA)26Sor locus with a loxP-flanked STOP cassette preventing transcription of the downstream optimized tetracycline-controlled transactivator protein. Applying both Cre-lox and Tet-Off technologies, these mutant mice may be useful for generating compound mutant mice in which expression of a tetracycline-responsive promoter element-driven gene of interest can be both directed to the cell types defined by the chosen Cre recombinase expression, as well as turned off by the addition of tetracycline (or its analog doxycycline).
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Gt(ROSA)26Sortm1(tTA)Roos
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Allele Name
targeted mutation 1
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Allele Attributes
Conditional ready (e.g. floxed); Transactivator
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Gene Symbol
Gt(ROSA)26Sor
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Gene Name
gene trap ROSA 26
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Chromosome
6
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Expressed Genes
tTA, tetracycline-controlled transactivator, E. coli
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MGI Accession ID
3806275
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Site of Expression
Widespread expression of the tetracycline-controlled transactivator (tTA) protein is initiated after removal of an upstream loxP-flanked STOP cassette by Cre recombinase. Thus the expression pattern of tTA is defined by the expression pattern of Cre recombinase.
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Strain of Origin
129P2/OlaHsd-Hprtb-m3
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Molecular Note
A targeting vector was designed to insert a loxP-flanked STOP cassette (containing a neomycin resistance cassette and transcriptional STOP element) just upstream of a modified/optimized form of tetracycline controlled transactivator ("mtTA") into the Gt(ROSA)26Sor locus. and chimeric mice were established.
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HUSBANDRY
Suggested Controls
C57BL/6J
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Breeding Considerations
When maintaining a live colony, homozygous mice may be bred together.
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For Research Use Only.
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