Online Inquiry
B6.Cg-Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/J
Cat. No.: CEMM-07250076
Common Name: Ai6; Ai6(RCL-ZsGreen)
Ai6 is a Cre reporter allele designed to have a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven enhanced green fluorescent protein variant (ZsGreen1) - all inserted into the Gt(ROSA)26Sor locus. Ai6 mice express robust ZsGreen1 fluorescence following Cre-mediated recombination.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze
show more
close
|
| Allele Name |
targeted mutation 6
show more
close
|
| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
show more
close
|
| Gene Symbol |
Gt(ROSA)26Sor
show more
close
|
| Gene Name |
gene trap ROSA 26
show more
close
|
| Chromosome |
6
show more
close
|
| Expressed Genes |
GFP, Green Fluorescent Protein
show more
close
|
| MGI Accession ID | show more close |
| Site of Expression |
A floxed STOP prevents transcription of enhanced green fluorescent protein (ZsGreen1) until mice are bred to a Cre strain. Resulting offspring express ZsGreen1 in a pattern dictated by the Cre promoter.
show more
close
|
| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
show more
close
|
| Molecular Note |
The Rosa-CAG-LSL-ZsGreen1-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), ZsGreen1 sequence (from pZsGreen1-N1 (Clontech); a human codon optimized/enhanced green fluorescent protein engineered for brighter fluorescence and higher expression in mammalian cells), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a polyA signal, and an attB/attP-flanked PGK-FRT Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.
show more
close
|
| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
show more
close
|
| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred.
show more
close
|
| Breeding Strategy |
Homozygote x Homozygote
show more
close
|
For Research Use Only.
Related Products