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B6.Cg-H2az2Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J
Cat. No.: CEMM-07250152
Common Name: Wnt-1/GAL4/cre-11
Wnt-1/GAL4/cre-11 transgenic mice were created by co-injection of both the pWEXP2-GAL4 transgene (Wnt1-GAL4) and the pWEXP3C-cre transgene (Wnt1-Cre). Transgenic mice have the wingless-related MMTV integration site 1 (Wnt1) promoter/regulatory sequences directing expression of both Cre recombinase and the GAL4 transcriptional activator to midbrain and developing neural tube. Wnt-1/GAL4/cre-11 mice exhibit transgenic overexpression of Wnt1, both within and outside of the expected pattern of expression, which results in increased activation of WNT signaling, along with midbrain enlargement and accompanying loss of midbrain dopaminergic neurons. Further, it was reported that the Wnt1-cre transgene integrated into chromosome 11 causing a 31,283 bp deletion in the H2afv locus. In, founder line 1 had a copy number of greater than 5.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
H2az2Tg(Wnt1-cre)11Rth
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| Allele Name |
transgene insertion 11
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| Allele Attributes |
Recombinase-expressing
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| Gene Symbol |
H2az2
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| Gene Name |
H2A.Z histone variant 2
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| Chromosome |
11
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| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
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| MGI Accession ID | show more close |
| Site of Expression |
Embryonic neural tube, midbrain, dorsal and ventral midlines of the midbrain and caudal diencephalon, the mid-hindbrain junction and dorsal spinal cord
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| Strain of Origin |
(C57BL/6J x CBA/J)F1/J
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| Molecular Note |
The Wnt1 promoter preceded the Cre recombinase coding sequence which was followed downstream by the Wnt1 enhancer. The Wnt1 regulatory sequences initially direct expression in the midbrain. After neural tube closure, expression occurs in the dorsal and ventral midlines of the midbrain and caudal diencephalon, the midbrain-hindbrain junction and in the dorsal spinal cord. Line 11 inserted into the gene at 6425500-6456783 (Build GRCm38/mm10) resulting in a 31,283 bp deletion. Founder line x has a copy number of greater than 5.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J Noncarrier
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| Breeding Considerations |
This strain has two co-injected transgenes that segregate together. Transgenic mice were bred to C57BL/6J inbred mice for many generations to establish this congenic strain. When maintaining the live congenic colony, transgenic carrier mice (harboring both co-injected transgenes) may be bred to non-carrier (wildtype) mice from the colony or to C57BL/6J inbred mice. The donating investigator reports that homozygous mice may be lethal as some offspring from transgenic carrier parents die around two months of age.
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For Research Use Only.
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