Products

Online Inquiry

B6;129S6-Gt(ROSA)26Sortm1(rtTA2S*M2)Whsu/J

Cat. No.: CEMM-07250332

This R26STOPrtTA mutant mouse strain combines two popular conditionally-regulated expression methodologies (Tet Expression and Cre Recombinase) providing a tool for doxycycline-inducible spatiotemporal and lineage specific expression of genes.
Inquiry
Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Gt(ROSA)26Sortm1(rtTA2S*M2)Whsu
show more close
Allele Name
targeted mutation 1
show more close
Allele Attributes
Conditional ready (e.g. floxed); Transactivator
show more close
Gene Symbol
Gt(ROSA)26Sor
show more close
Gene Name
gene trap ROSA 26
show more close
Chromosome
6
show more close
MGI Accession ID
3584142
show more close
Site of Expression
When mice carrying this allele are bred to mice that express Cre recombinase, the resulting offspring will express rtTAS*M2, reverse tetracycline regulated transactivator, in the Cre expressing cells. When bred to mice that also carry a gene of interest under the regulatory control of a tetracycline-responsive promoter element (tetO), expression of the gene of interest may be regulated by the tetracycline analog, doxycycline (dox). In the presence of dox, transcription of the target gene is induced in cells where rtTA is expressed.
show more close
Strain of Origin
129S6/SvEvTac
show more close
Molecular Note
A synthetic modified version of the rtTA gene preceded by a loxP flanked STOP sequence was inserted into the Gt(ROSA)26Sor locus. This version of rtTA contains 5 amino acid changes in the TetR moiety (S12G, E19G, A56P, D148E, and H179R) and a synthetic optimized transactivating domain. This rtTA has reduced basal activity and enhanced doxycycline sensitivity compared to wild-type rtTA.
show more close
HUSBANDRY
Suggested Controls
B6129SF1/J
show more close
Breeding Considerations
When maintaining a live colony, these mice can be bred as homozygotes.
show more close
For Research Use Only.
Related Products