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B6.Cg-Gt(ROSA)26Sortm1.3(CAG-tdTomato, -EGFP)Pjen/J

Cat. No.: CEMM-07250621

Common Name: RC::FLTG
The RC::FLTG dual-recombinase responsive indicator allele is designed with a frt-flanked STOP and loxP-flanked tdTomato::STOP all preventing transcription of eGFP. Flp recombinase results in high tdTomato fluorescence, and further exposure to Cre recombinase results in robust eGFP fluorescence. The RC::FLTG allele and its derivatives are useful for fluorescent labeling of cells/tissues in embryonic or adult mice. These mice allow intersectional genetic fate mapping of different cell subpopulations defined by the overlap of two gene expression domains.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Gt(ROSA)26Sortm1.3(CAG-tdTomato,-EGFP)Pjen
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Allele Name
targeted mutation 1.3
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Allele Attributes
Conditional ready (e.g. floxed); Reporter
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Gene Symbol
Gt(ROSA)26Sor
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Gene Name
gene trap ROSA 26
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Chromosome
6
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Expressed Genes
RFP, Red Fluorescent Protein, coral; GFP, Green Fluorescent Protein
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MGI Accession ID
5617964
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Site of Expression
EGFP and tdTomato will be expressed in cells/tissues where promoters driving Cre recombinase and Flp recombinase are expressed.
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Strain of Origin
(129S6/SvEvTac x C57BL/6NCrl)F1
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Molecular Note
The targeting construct contains a a rox-flanked STOP, FRT flanked STOP and loxP-flanked tdTomato::STOP upstream of the eGFP fluorescent protein, all inserted into the Gt(ROSA)26Sor locus. Dre-mediated recombination removed the rox-flanked STOP.
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HUSBANDRY
Suggested Controls
C57BL/6J
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Breeding Considerations
When maintaining a live colony, homozygous mice may be bred together.
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Breeding Strategy
Homozygote x Homozygote
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For Research Use Only.
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