B6.129S6(Cg)-Nkx2-2^{tm4.1(cre/EGFP)Suss}/J

Nkx2-2^{tm4.1(cre/EGFP)Suss}

targeted mutation 4.1

Recombinase-expressing; Null/Knockout

Nkx2-2

NK2 homeobox 2

2

GFP/Cre, Green Fluorescent Protein/Cre recombinase fusion protein

5538250

Cre recombinase and EGFP are expressed in Nkx2-2-expressing cells of the central nervous system, pancreas, and intestine.

129S6/SvEvTac

A Cre/EGFP fusion gene and Frt-flanked hygro selection cassette were inserted using recombineering into an Nkx2-2 basal exchange vector such that Cre/EGFP was fused in-frame with the ATG start codon, replacing all but 25 base pairs of the first coding exon. This vector was co-injected with a Cre-expression vector into ES cells containing an Nkx2-2 (loxed cassette acceptor) allele. Correctly exchanged ES clones were used to produce chimeras which were bred to B6 mice to verify germline transmission. Animals were bred to ACTB-Flpe mice to excise the hygromycin marker.

C57BL/6J Wild-type from the colony

When maintaining a live colony, heterozygous mice may be bred together, to wildtype siblings, or to C57BL/6J inbred mice. Homozygotes have a neonatal lethal phenotype.

Wild-type x Heterozygote; Heterozygote x Wild-type