B6.Cg-Calb1^{tm1.1(folA/cre)Hze}/J

Calb1^{tm1.1(folA/cre)Hze}

targeted mutation 1.1

Recombinase-expressing; Inducible

Calb1

calbindin 1

4

GFP, Green Fluorescent Protein; cre/folA

5522769

Pattern correlates well with endogenous Calb1 expression: after trimethoprim induced Cre recombinase activity, Cre-inducible reporter allele expression is detected in scattered cells of the cortex, hippocampus, cerebellum and striatum, and restricted cell populations in thalamus and hypothalamus.

(129S6/SvEvTac x C57BL/6NCrl)F1

The targeting vector inserted a viral 2A oligopeptide (T2A, mediating ribosomal skipping) and a destabilized EGFP/Cre fusion gene (dgCre) inserted immediately downstream of the calbindin 1 translational STOP codon. The dgCre fusion gene (also called destabilized EGFP/Cre, DHFR-EGFP-Cre, hDHFR/EGFP/Cre or ecDHFR/EGFP/Cre) is an enhanced green fluorescent protein/Cre recombinase fusion gene with an N terminal fusion of the first 159 amino acids of the Escherichia coli K-12 strain chromosomal dihydrofolate reductase gene (DHFR or folA) harboring the G67S mutation and modified to also include the R12Y/Y100I destabilizing domain mutations. A PGK-neo-polyA cassette flanked by AttB and AttP sites was also part of the construct. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were bred to PhiC31-expressing mice to delete the AttB/AttP-flanked sequences and replace it with the recombined AttB/AttP site (AttL). Trimethoprim-treatment is required to stabilize the Cre protein. No EGFP is detected with or without induction.

C57BL/6J Wild-type from the colony

When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice. The phenotype of homozygous mice has not yet been determined.

Wild-type x Heterozygote; Heterozygote x Wild-type