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B6(C3)-Agtr1atm1.1(cre)Ekrs/J

Cat. No.: CEMM-07250814

Common Name: AT1aR-Cre knock-in
AT1aR-Cre mice express Cre recombinase and zsGreen under the direction of the Agtr1a promoter and may be useful when studying the regulation of stress responses.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Agtr1atm1.1(cre)Ekrs
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Allele Name
targeted mutation 1.1
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Allele Attributes
Recombinase-expressing; Reporter
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Gene Symbol
Agtr1a
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Gene Name
angiotensin II receptor
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Chromosome
13
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Expressed Genes
GFP, Green Fluorescent Protein; Cre, Cre recombinase, bacteriophage P1
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MGI Accession ID
5906996
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Site of Expression
When bred with mice containing loxP-flanked sequence, Cre-mediated recombination will result in deletion of the floxed sequences in the Cre-expressing cells of the offspring.
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Strain of Origin
C57BL/6J
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Molecular Note
A targeting construct was designed to insert a Cre Recombinase gene and a zsGreen (enhanced green fluorescence protein) just upstream of the 3'UTR of the angiotensin II receptor, type 1a (Agtr1a) gene. Viral P2A oligopeptides, from porcine teschovirus-1, were inserted upstream and downstream of the Cre gene to enable ribosomal skipping. A FRT flanked neomycin resistance (neo) cassette was placed downstream of the 3'UTR. This construct was electroporated into C57BL/6J embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric mice were bred to FLP (FLPo) recombinase expressing mice to delete the neo cassette.
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HUSBANDRY
Suggested Controls
C57BL/6J
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Breeding Considerations
When maintaining a live colony homozygous mice may be bred together.
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Breeding Strategy
Homozygote x Homozygote
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For Research Use Only.
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