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B6.Cg-Eef1a1tm1Rck/J
Cat. No.: CEMM-07250792
Common Name: EEF1A1-LSL.EGFPL10
Removal of this mouse colony is imminent. If live mice are needed for your studies, it is advised that they be ordered immediately. After removal, the mice will be available from a cryorecovery.Cre-mediated excision of a floxed STOP enables tissue-specific expression of EGFP-tagged ribosomal protein that enables transcriptional profiling using TRAP technology. Expression of EGFP/RPL10a is driven by Eef1a1, a strong promoter that is widely expressed at high levels in the nervous system and in most tissues.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Eef1a1tm1Rck
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Eef1a1
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| Gene Name |
eukaryotic translation elongation factor 1 alpha 1
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| Chromosome |
9
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| Expressed Genes |
GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
Cre-mediated recombination of this allele results in broad expression of the EGFPL10 protein.
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| Strain of Origin |
B6(Cg)-Tyrc-2J
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| Molecular Note |
The cDNA encoding a fusion of an EGFP protein with the L10 (large subunit ribosomal protein L10) was inserted into a vector downstream of a floxed 'STOP' cassette and a neomycin selection marker and upstream of a polyadenylation signal. Homologous recombination in ES cells inserted this sequence into the first intron of Eef1a1. Cre-mediated recombination of this allele results in expression of the EGFPL10 protein.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
Heterozygotes are viable and fertile. Homozygotes are not viable.
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| Breeding Strategy |
Wild-type x Heterozygote; Heterozygote x Wild-type
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For Research Use Only.
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