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B6(129S6)-P2ry12em1(icre/ERT2)Tda/J
Cat. No.: CEMM-07250992
Common Name: P2ry12-CreER
P2ry12-CreER mice express a tamoxifen-inducible Cre driven by the endogenous purinergic receptor P2Y, G-protein coupled 12 (P2ry12) promoter. These mice are a Cre-lox tool, allowing inducible Cre recombination in P2ry12-expressing cells/tissues (primarily microglia), and may be useful in studying neuroinflammation, neurodegenerative disease, and immune response.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
P2ry12em1(icre/ERT2)Tda
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| Allele Name |
endonuclease-mediated mutation 1
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| Allele Attributes |
Recombinase-expressing; Inducible
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| Gene Symbol |
P2ry12
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| Gene Name |
purinergic receptor P2Y
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| Chromosome |
3
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| MGI Accession ID | show more close |
| Strain of Origin |
C57BL/6N
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| Molecular Note |
The allele was Created using CRISPR/Cas9 endonuclease-mediated genome editing to target the stop codon of the P2ry12 locus on chromosome 3. The Cas9-RNP complex and a 2A-iCreERT2 dsDNA (encoding a viral 2A oligopeptide that mediates ribosomal skipping and the iCreER fusion gene with ~1.5 kbp homology arms) were injected into the pronucleus of C57BL/6N mouse embryos, followed by adoptive embryo transfer. Founder mice and progeny were sCreened by PCR, and proper genomic integration was verified by Southern blot and genomic sequencing. Founders were bred to C57BL/6J mice for germline transmission.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
Both heterozygous and homozygous mice are viable and fertile with normal breeding, and no reported gross phenotypic or behavioral abnormalities. When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice. Alternatively, homozygous mice may be bred together.
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For Research Use Only.
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