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B6;129S6-Gt(ROSA)26Sortm1(CAG-cas9*/EP300*)Gers/J
Cat. No.: CEMM-07250940
Common Name: Rosa26-LSL-dCas9-p300; R26-LSL-dCas9p300 Core
This strain is currently unavailable due to replenishing of cryopreserved stocks. Interested customers who register interest will be contacted when the strain is again available Rosa26-LSL-dCas9-p300 knock-in mice (also called R26-LSL-dCas9p300 Core) have a loxP-flanked STOP cassette preventing expression of the dCas9p300 Core fusion protein - a codon-optimized, nuclease-deficient, catalytically dead Cas9 protein (dCas9) fused to the catalytic core of the human acetyltransferase p300. Following Cre recombinase and introduction of a single guide RNA (sgRNA), dCas9p300 Core binds to the target DNA sequence/locus, and that locus is subsequently activated by the p300 core domain. These mice allow Cre-inducible, RNA-guided transcriptional activation of target genes from promoters and both proximal and distal enhancers in a wide range of cellular processes.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(CAG-cas9*/EP300*)Gers
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Epitope tag; Endonuclease
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| MGI Accession ID | show more close |
| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette, a dCas9 sequence (dCas9-NLS-p300; see below), a 1xFLAG epitope tag, a WPRE, a bGH polyA signal and an attB-PGK-FRT Neo-polyA-attP cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The dCas9 used here is composed of a codon-optimized, nuclease-deficient Streptococcus pyogenes Cas9 protein (dCas9) DNA fused at its C-terminus to a nucleoplasmin bipartite nuclear localization signal and the catalytic histone acetyltransferase (HAT) core domain of the human E1A-associated protein p300 (EP300).
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
Wild-type from the colony
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| Breeding Considerations |
Prior to introduction of Cre recombinase, mice heterozygous or homozygous for the Rosa26-LSL-dCas9-p300 allele are viable and fertile with normal fertility, and no gross physical or behavioral abnormalities. When maintaining a live colony, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice. Alternatively, homozygous mice may be bred together.
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For Research Use Only.
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