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B6;129-Ghrhtm1.1(cre)Mgmj/J
Cat. No.: CEMM-07250845
Common Name: GhrhCre
GhrhCre knock-in mice may be useful for studying energy balance and glucose homeostasis.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Ghrhtm1.1(cre)Mgmj
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| Allele Name |
targeted mutation 1.1
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| Allele Attributes |
Recombinase-expressing
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| Gene Symbol |
Ghrh
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| Gene Name |
growth hormone releasing hormone
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| Chromosome |
2
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| MGI Accession ID | show more close |
| Site of Expression |
Cre recombinase is expressed in areas of the hypothalamus, including the arcuate nucleus, with minimal expression in a few regions in the midbrain and hindbrain.
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| Strain of Origin |
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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| Molecular Note |
A targeting construct was designed to replace the stop codon of the growth hormone releasing hormone (Ghrh) gene with a viral P2A oligopeptide, from porcine teschovirus-1, fused to a Cre recombinase gene. A FRT flanked neomycin resistance (neo) cassette inserted downstream. The construct was electroporated into R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric mice were bred with B6.129S4-Gt(ROSA)26Sor/J mice to remove the neo cassette.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred together.
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| Breeding Strategy |
Heterozygote x Heterozygote
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For Research Use Only.
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