Online Inquiry
B6;129S2-Syngap1tm2Geno/RumbJ
Cat. No.: CEMM-07250725
Common Name: SYNGAP1lx-st (Cre-conditional rescue)
The SYNGAP1lx-st conditional rescue knock-in allele has a loxP-flanked neo-STOP cassette upstream of exon 6 of the synaptic RasGAP (SynGAP) gene; resulting in disrupted expression of full-length SynGAP and expression of a truncated/inactive SynGAP protein. These mice allow Cre recombinase-inducible restoration of full-length SynGAP expression. SYNGAP1lx-st mice may be useful Cre-lox studies of synapse development (specifically in dendritic spine), cognitive and behavioral maturation, intellectual disability and autism spectrum disorder.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Syngap1tm2Geno
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| Allele Name |
targeted mutation 2
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| Allele Attributes |
Inserted expressed sequence; Humanized sequence
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| Gene Symbol |
Syngap1
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| Gene Name |
synaptic Ras GTPase activating protein 1 homolog (rat)
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| Chromosome |
17
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| Expressed Genes |
Syngap1, synaptic Ras GTPase activating protein 1 homolog (rat), mouse, laboratory
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| MGI Accession ID | show more close |
| Strain of Origin |
129S2/SvPas
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| Molecular Note |
A floxed neo cassette was inserted into intron 5. Western blot analysis confirmed reduced protein expression in heterozygous samples. Cre-mediated recombination is required to remove the neo cassette and restore protein expression.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
Wild-type from the colony
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| Breeding Considerations |
Homozygous mice exhibit complete postnatal lethality between 2-5 days of age. Heterozygous mice develop profound cognitive and behavioral abnormalities grossly demonstrable ~21 days of age. The donating investigator breeds heterozygous mice to wildtype mice from the colony, using both sexes as the heterozygote. When maintaining a live colony, Repository, heterozygous mice may be bred to wildtype mice from the colony or to C57BL/6J inbred mice.
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| Breeding Strategy |
Wild-type x Heterozygote; Heterozygote x Wild-type
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For Research Use Only.
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