Online Inquiry
B6.Cg-Gt(ROSA)26Sortm2(CAG-PA-GFP)Rmpl/J
Cat. No.: CEMM-07251030
Common Name: H2B::PaGFP
H2B::PaGFP mice carry a photoactivatable histone H2B/green fluorescent protein fusion gene (Pa-GFP) behind a floxed STOP cassette (LSL). In the presence of Cre recombinase and photoactivation, bright nuclear fluorescence can be detected, allowing for the observation and measurement of nuclear and chromatin dynamics in vivo.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm2(CAG-PA-GFP)Rmpl
show more
close
|
| Allele Name |
targeted mutation 2
show more
close
|
| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
show more
close
|
| Gene Symbol |
Gt(ROSA)26Sor
show more
close
|
| Gene Name |
gene trap ROSA 26
show more
close
|
| Chromosome |
6
show more
close
|
| MGI Accession ID | show more close |
| Strain of Origin |
(C57BL/6 x 129P2/OlaHsd)F1
show more
close
|
| Molecular Note |
The targeting vector is designed with (from 5' to 3') the CMV-IE enhancer/chicken beta-actin hybrid promoter (CAG; from the pCAGGS vector), an FRT site, a STOP cassette flanked by loxP sites, a photoactivatable green fluorescent protein (PA-GFP) fused to the human histone H2B sequence, and a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability).
show more
close
|
| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
show more
close
|
| Breeding Considerations |
Both heterozygous and homozygous mice are viable and fertile with normal breeding, and no reported gross phenotypic or behavioral abnormalities. When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice. Alternatively, homozygous mice may be bred together.
show more
close
|
| Breeding Strategy |
Heterozygote x Heterozygote
show more
close
|
For Research Use Only.
Related Products