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B6;129S-Trhtm1.1(cre)Mjkr/LowlJ

Cat. No.: CEMM-07250919

Common Name: Trh-ires-cre
Trh-ires-Cre knock-in mice express Cre recombinase under direction of the thyrotropin releasing hormone promoter in the paraventricular hypothalamus making them useful for cell-specific neuron mapping. Unlike conventional cre-expressing mice, the donating investigator reports that the early embryonic expression of Trh, and thus the early embryonic expression of Cre, results in deletion of the floxed sequences in all cells of adult mice. However, these mice are very useful when used in conjunction with injection of Cre-dependent AAVs into brains of adult Trh-ires-Cre mice.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Trhtm1.1(cre)Mjkr
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Allele Name
targeted mutation 1.1
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Allele Attributes
Recombinase-expressing
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Gene Symbol
Trh
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Gene Name
thyrotropin releasing hormone
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Chromosome
6
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MGI Accession ID
5562874
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Site of Expression
Anterior pituitary gland
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Strain of Origin
129S6/SvEvTac
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Molecular Note
Using recombineering methods, a selection cassette containing an internal ribosomal entry sequence (IRES) linked to Cre and an FRT flanked kanamycin gene was inserted immediately downstream of the stop codon in exon 2 of the Trh gene in a BAC. This construct was used to isolate a targeting plasmid with the Cre-kanamycin cassette with upstream and downstream Trh homology arms. ES cells were targeted and used for blastocyst injection. Chimeras were bred for germline transmission. The selection marker was excised by breeding to Flp deleter animals, however, a partial neo (164 bp fragment of the 3' end of neo) remains between the IRES-Cre and polyA sequences.
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HUSBANDRY
Suggested Controls
B6129SF2/J
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Breeding Considerations
When maintaining a live colony, homozygous mice may be bred together.
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Breeding Strategy
Heterozygote x Heterozygote
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For Research Use Only.
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