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B6.129S4-Gt(ROSA)26Sortm1Sor/J

Cat. No.: CEMM-07250007

Common Name: R26R
Genetic Background: C57BL/6J
These Gt(ROSA)26Sortm1Sor targeted mutant mice carry a loxP flanked neo cassette upstream of a β-galactosidase (lacZ) sequence. Removal of the neo cassette by cre recombination results in lacZ expression in cre-expressing tissues of the offspring. These mutant mice may be used as a Cre-reporter strain to test the tissue/cellular expression pattern of cre expressing mice.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Gt(ROSA)26Sortm1Sor
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Allele Name
targeted mutation 1
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Allele Attributes
Conditional ready (e.g. floxed); Reporter
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Gene Symbol
Gt(ROSA)26Sor
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Gene Name
gene trap ROSA 26
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Chromosome
6
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Expressed Genes
lacZ, beta-galactosidase, E. coli
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MGI Accession ID
1861932
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Site of Expression
When crossed to a Cre recombinase-expressing strain, LacZ expression is observed in the Cre-expressing tissues
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Strain of Origin
129S4/SvJaeSor
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Molecular Note
A targeting vector was designed from the original gene trap strain, ROSA beta-geo26, to include a splice acceptor sequence (SA), a neo expression cassette flanked by loxP sites, a lacZ gene, and a polyadenylation (bpA) sequence inserted at a unique Xba1 site approximately 300-bp 5' of the original gene-trap integration site. In addition, a triple polyadenylation sequence was added to the 3' end of the neo expression cassette to prevent transcriptional read-through. Presence of the floxed neo cassette prevents lacZ expression. When crossed with a Cre transgenic strain, lacZ is expressed in all cells/tissue where Cre is expressed.
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HUSBANDRY
Suggested Controls
C57BL/6J
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Breeding Considerations
Homozygous mice are viable and fertile. When maintaining a live colony, homozygous mice may be bred together. Coat color expected from breeding is Black.
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Breeding Strategy
Homozygote x Homozygote
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For Research Use Only.
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