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B6;129X1-Gt(ROSA)26Sortm2(ATF4)Myz/J
Cat. No.: CEMM-07250727
Common Name: Rosa26-ATF4loxtg
Rosa26-ATF4loxtg mutant mice carry a floxed stop/FLAG-tagged human ATF4 cassette knocked into the mouse Gt(ROSA)26Sor gene. Cre-mediate excision of the floxed stop enables site-directed expression of FLAG-tagged human ATF4 protein.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm2(ATF4)Myz
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| Allele Name |
targeted mutation 2
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| Allele Attributes |
Conditional ready (e.g. floxed); Inserted expressed sequence; Humanized sequence
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
ATF4, activating transcription factor 4, human
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| MGI Accession ID | show more close |
| Site of Expression |
Following Cre recombination, FLAG-tagged human ATF4 protein is expressed in Cre-expressing tissues.
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| Strain of Origin |
(C57BL/6J x 129X1/SvJ)F1
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| Molecular Note |
The targeting vector containing a loxP site, PGK-puromycin-pA cassette (in reverse transcriptional orientation), 4x SV40-pA STOP, loxP, and FLAG-human ATF4-WPRE-BGH-pA cassette sequence is introduced to the Gt(ROSA)26Sor gene via a FLP recombinase-mediated cassette exchange method.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J Wild-type from the colony
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| Breeding Considerations |
Mice that are heterozygous or homozygous for the floxed-Stop are viable and fertile.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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