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B6;129S-Gt(ROSA)26Sortm2(CAG-cre*)Yzwa/J
Cat. No.: CEMM-07250955
Common Name: Rosa26PA-Cre B4
Rosa26PA-Cre B4 mice express a photoactivatable Cre recombinase (PA-Cre 3.0) following exposure to FLP recombinase. Specifically, FLP-mediated removal of the frt-flanked STOP cassette results in expression of the individual split Cre segments (nMag::CreN59 and pMag::CreC60). Subsequent illumination with blue-light causes nMag-pMag dimerization (Magnets-opti dimerization), which brings the split Cre segments together to form a functional Cre recombinase molecule. This allows for highly efficient DNA recombination in an internal organ of living mice through noninvasive external blue light illumination, without unintended leakiness in the dark condition.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm2(CAG-cre*)Yzwa
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| Allele Name |
targeted mutation 2
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| Allele Attributes |
Conditional ready (e.g. floxed); Recombinase-expressing
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| MGI Accession ID | show more close |
| Strain of Origin |
Not Specified
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| Molecular Note |
The targeting vector was designed with (from 5' to 3') a ubiquitously expressed CAG (CMV enhancer/chicken beta-actin) promoter, a FRT flanked STOP cassette (containing a neomycin resistance (neo) cassette and a polyadenylation [pA] site). A PA-Cre 3.0 followed by a pA site was placed downstream of the FRT flanked STOP cassette. PA-Cre 3.0 consists of a split Cre (59/60) with each fragment complemented with nMag or pMag (respectively) and a nuclear localization sequence (NLS) (CreN59-nMag-NLS and NLS-pMag-CreC60), separated by P2A self-cleaving peptide sequence (2A). This targeting vector was inserted into the Gt(ROSA)26Sor locus. The construct was electroporated into 129Sv/Ev x C57BL/6 derived embryonic stem (ES) cells (clone B4) and correctly targeted ES cells were injected into blastocysts.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
B6129SF2/J
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| Breeding Considerations |
When maintaining a live colony, heterozygous mice may be bred to non-carrier (wildtype) mice from the colony, or to C57BL/6J inbred mice. The donating investigator has not attempted to make this strain homozygous.
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| Breeding Strategy |
Wild-type x Heterozygote; Heterozygote x Wild-type
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For Research Use Only.
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