Online Inquiry
B6.129P2(129S4)-Hprttm9(Ple178-EGFP/cre)Ems/Mmjax
Cat. No.: CEMM-07250128
These Ple178-EGFPCre;mEMS756 mice have the Ple178-EGFPCre transgene targeted as a single copy "knock-in" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. As the promoter/regulatory regions of the human regulator of G-protein signaling 16 (RGS16) gene direct expression of an EGFPCre fusion protein to hippocampus, thalamus, forebrain, cortex, brainstem, cerebellum, and retina, these Ple178-EGFPCre;mEMS756 mice may be useful in studying RGS16-expressing cells in the brain and diseases affecting the brain.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Hprttm9(Ple178-EGFP/cre)Ems
show more
close
|
| Allele Name |
targeted mutation 9
show more
close
|
| Allele Attributes |
Recombinase-expressing; Reporter
show more
close
|
| Gene Symbol |
Hprt
show more
close
|
| Gene Name |
hypoxanthine guanine phosphoribosyl transferase
show more
close
|
| Chromosome |
X
show more
close
|
| Expressed Genes |
GFP, Green Fluorescent Protein; Cre, Cre recombinase, bacteriophage P1
show more
close
|
| MGI Accession ID | show more close |
| Site of Expression |
Expression of an EGFPCre fusion protein is directed to hippocampus, thalamus, forebrain, cortex, brainstem, cerebellum, and retina
show more
close
|
| Strain of Origin |
129P2/OlaHsd
show more
close
|
| Molecular Note |
The Ple178-EGFPCre transgene (pEMS1090) was designed with the 864 bp Ple178 minipromoter (RGS16-D; derived from an intronic candidate regulatory region and a subsection of the human regulator of G-protein signaling 16 (RGS16) promoter) upstream of a minimal F5 mutant-FRT site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal FRT site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2).
show more
close
|
| HUSBANDRY | |
|---|---|
| Breeding Considerations |
The donating investigator recommends maintaining this strain by breeding heterozygous females with C57BL/6J inbred males.
show more
close
|
For Research Use Only.
Related Products