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B6.Cg-Tg(Plp1-cre/ERT)3Pop/J

Cat. No.: CEMM-07250031

Common Name: PLP/creERT
These transgenic mice have a tamoxifen inducible Cre-mediated recombination system driven by the mouse Plp1, proteolipid protein (myelin) 1 promoter. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions. Tamoxifen administration allows for ablation of predetermined genes in oligodendrocytes and Schwann cells, and will also induce Cre recombination in developing embryos of treated mothers and in cultured cells derived from transgenic mice.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Tg(Plp1-cre/ERT)3Pop
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Allele Name
transgene insertion 3
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Allele Attributes
Recombinase-expressing; Inducible
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Gene Symbol
Tg(Plp1-cre/ERT)3Pop
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Gene Name
transgene insertion 3
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Chromosome
UN
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Expressed Genes
Cre, Cre recombinase, bacteriophage P1; ESR1, estrogen receptor 1, human
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MGI Accession ID
2450391
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Site of Expression
Tamoxifen inducible Cre recombinase protein fused to mutant form of the mouse estrogen receptor ligand binding domain; expression in oligodendrocytes and Schwann cells
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Strain of Origin
(C57BL/6 x DBA/2)F1
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Molecular Note
The transgenic construct encodes a tamoxifen-inducible fusion protein consisting of Cre recombinase and a mutated human estrogen receptor ligand-binding domain cloned next to a Plp promoter. The 2.5 kb Plp regulatory region includes 5' flanking DNA, exon 1, and intron 1. Transgene expression was confirmed by RT-PCR and Western blot analysis. The mouse Plp promoter was used to drive transgene expression in myelinating cells.
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HUSBANDRY
Suggested Controls
C57BL/6J
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Breeding Considerations
When maintaining a live colony, these mice are bred as hemizygotes.
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Breeding Strategy
Hemizygote x +/+ sibling
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For Research Use Only.
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