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STOCK Gad2tm2(cre)Zjh/J
Cat. No.: CEMM-07250204
Common Name: Gad2-IRES-Cre
Gad2-IRES-Cre knock-in mice have Cre recombinase expression directed to GAD2 positive neurons. These mice may be used to generate conditional mutations for studying GAD2 cell populations.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gad2tm2(cre)Zjh
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| Allele Name |
targeted mutation 2
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| Allele Attributes |
Recombinase-expressing
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| Gene Symbol |
Gad2
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| Gene Name |
glutamic acid decarboxylase 2
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| Chromosome |
2
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| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
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| MGI Accession ID | show more close |
| Site of Expression |
When Gad2-IRES-Cre mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the Gad2-expressing cells (GAD2 positive neurons) of the offspring.
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| Strain of Origin |
(C57BL/6 x 129S4/SvJae)F1
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| Molecular Note |
A targeting vector was designed to insert an internal ribosome entry site (IRES), a Cre recombinase sequence, an SV40 polyA signal, and an FRT flanked neo cassette into the 3' untranslated region (after the translational termination site) of the Gad2 (glutamic acid decarboxylase 2) gene. This construct was electroporated into C57BL6/S129 hybrid embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient tetraploid blastocysts. Chimeric mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10)) to remove the selection cassette. The FLPe transgene was subsequently bred out of the background.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
B6129SF2/J
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| Breeding Considerations |
Homozygous mice are viable and fertile. When maintaining a live colony Repository, homozygous mice may be bred together.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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