Online Inquiry
STOCK Trp53tm1Brd Brca1tm1Aash Tg(LGB-cre)74Acl/J
Cat. No.: CEMM-07250230
Common Name: BLG-Cre; Brca1F22-24/F22-24; p53+/-
These mice carry the beta-lactoglobulin Cre (BLG-Cre) transgene, are homozygous for floxed exons 22-24 of the breast cancer 1 (Brca1) allele, and are heterozygous for p53 tumor-suppressor gene (Trp53) deficiency. This strain may be useful for studying human basal-like cancer and breast cancer, as well as providing a useful tool for testing new therapeutics.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Brca1tm1Aash
show more
close
|
| Allele Name |
targeted mutation 1
show more
close
|
| Allele Attributes |
Conditional ready (e.g. floxed); No functional change
show more
close
|
| Gene Symbol |
Brca1
show more
close
|
| Gene Name |
breast cancer 1
show more
close
|
| Chromosome |
11
show more
close
|
| MGI Accession ID | show more close |
| Site of Expression |
Mammary epithelial cells
show more
close
|
| Strain of Origin |
(129X1/SvJ x 129S1/Sv)F1-Kitl+
show more
close
|
| Molecular Note |
Exons 22-24 were replaced with a floxed sequence containing cDNA for exons 22-24 with an in frame myc epitope and a bovine growth hormone poly A sequence. This vector allows conditional excision of the second, terminal BRCT domain. Downstream of the floxed sequence, a splice acceptor and PGK-neo were inserted.
show more
close
|
| HUSBANDRY | |
|---|---|
| Breeding Considerations |
To induce the tumor development in these mice, the donating investigator reports that BLG-Cre; Brca1F22-24/F22-24; p53+/- mice should be allowed to go through two rounds of pregnancy and then set aside to allow Cre activation and the loss of Brca1 function.
show more
close
|
| Breeding Strategy |
Heterozygous Homozygous Noncarrier x Wildtype Homozygous Hemizygous AND Wildtype Homozygous Noncarrier x Heterozygous Homozygous Hemizygous
show more
close
|
For Research Use Only.
Related Products