STOCK Tg(Thy1-Brainbow3.1)3Jrs/J

Tg(Thy1-Brainbow3.1)3Jrs

transgene insertion 3

Reporter

Tg(Thy1-Brainbow3.1)3Jrs

transgene insertion 3

UN

OFP, Orange Fluorescent Protein, jelly fish; GFP, Green Fluorescent Protein; RFP, Red Fluorescent Protein, coral; YFP, Yellow Fluorescent Protein, jellyfish

5469425

Fluorescence is detected in motor neurons, cortical neurons, hippocampal neurons, cerebellar neurons, and cerebellar Purkinje cells.

C57BL/6J x CBA

The Thy1-Brainbow 3.1 transgene was designed by Drs. Dawen Cai and Joshua R.Sanes (Harvard University) with the mouse Thy1 (thymus cell antigen 1, theta) regulatory elements surrounding four fluorescent protein (Yellow fluorescent protein (Phialidium sp); Orange fluorescent protein: Green fluorescent protein; Far-red fluorescent protein) sequences uniquely flanked with pairs of incompatible lox sites. Specifically, this Brainbow 3.1 coding region contained (from 5' to 3') a loxP site, lox2272 site, loxN site, non-fluorescent nuclear localized derivative of phi-YFP (phiNFPnls), polyA sequence, loxP site, farnesylated mOrange2, polyA sequence, lox2272 site, farnesylated EGFP, polyA sequence, loxN site, farnesylated mKate2, polyA sequence. Line 3 was generated.

Noncarrier

When maintaining a live colony, hemizygous mice are bred with wildtype (non-carrier) mice from the colony or with C57BL/6J inbred mice. The Donating Investigator has not attempted to make the strain homozygous to date.