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STOCK Tg(Ccna2-lacZ, -EGFP)1Sev/J
Cat. No.: CEMM-07250796
Common Name: CyclinA2-LacZ-EGFP (or CyclinA2-floxed:LacZ-GFP)
The CyclinA2-lacZ-EGFP BAC transgenic mouse line (or CyclinA2-floxed:LacZ-GFP) is a robust and sensitive reporter of cell cycle activity in any cell type of interest - enabling the visualization of cell cycle activity in specific cell types. These CyclinA2-fusion reporter mice are designed to express CyclinA2-lacZ (CyclinA2-βgal) prior to Cre recominase exposure, and CyclinA2-EGFP following Cre recombination.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Tg(Ccna2-lacZ,-EGFP)1Sev
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| Allele Name |
transgene insertion 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Tg(Ccna2-lacZ,-EGFP)1Sev
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| Gene Name |
transgene insertion 1
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| Chromosome |
UN
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| Expressed Genes |
Ccna2, cyclin A2, mouse, laboratory; lacZ, beta-galactosidase, E. coli; GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
Upon exposure to Cre recombinase, EGFP fluorescence is seen in Cre expressing cells of offspring.
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| Strain of Origin |
BALB/c and C57BL/6
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| Molecular Note |
The BAC clone, RP23-297G4, containing the entire cyclin A2 locus (Ccna2), as well as ~80 kbp 3' (centromeric) flanking region and ~89 kbp 5' (telomeric) flanking region is modified by the insertion of a loxP::lacZ-3pA::loxP cassette followed by an EGFP cassette in-frame immediately before the Ccna2 stop codon. The centromeric region harbors the complete Exosc9 locus and at least three predicted genes, and the telomeric region includes the complete Bbs7 locus, one incomplete locus (3' portion of Trpc3) and at least one predicted gene. No other loci on the BAC were altered further. The transgene retains a chloramphenicol resistance cassette from the BAC sequences.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
Noncarrier
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| Breeding Considerations |
Hemizygous mice are viable and fertile with no reported gross physical or behavioral abnormalities. it has not been attempted to make this strain homozygous. When maintaining a live colony, hemizygous mice may be bred with wildtype (non-carrier) mice from the colony.
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| Breeding Strategy |
Hemizygote x Noncarrier; Noncarrier x Hemizygote
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For Research Use Only.
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