Online Inquiry
B6.Cg-Gt(ROSA)26Sortm3(CAG-EYFP)Hze/J
Cat. No.: CEMM-07250074
Common Name: Ai3; Ai3(RCL-EYFP)
These Ai3 mice harbor a targeted mutation of the Gt(ROSA)26Sor locus with a loxP-flanked STOP cassette preventing transcription of a CAG promoter-driven enhanced yellow fluorescent protein (EYFP), and may be useful as a Cre reporter strain. EYFP is expressed following Cre-mediated recombination.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm3(CAG-EYFP)Hze
show more
close
|
| Allele Name |
targeted mutation 3
show more
close
|
| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
show more
close
|
| Gene Symbol |
Gt(ROSA)26Sor
show more
close
|
| Gene Name |
gene trap ROSA 26
show more
close
|
| Chromosome |
6
show more
close
|
| Expressed Genes |
YFP, Yellow Fluorescent Protein, jellyfish
show more
close
|
| MGI Accession ID | show more close |
| Site of Expression |
A floxed STOP prevents transcription of EYFP until mice are bred to a Cre strain. Resulting offspring express EYFP in a pattern dictated by the Cre promoter.
show more
close
|
| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
show more
close
|
| Molecular Note |
The Rosa-CAG-LSL-EYFP-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), an enhanced yellow fluorescent protein sequence (from pcDNA6.2/N-YFP-DEST), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a polyA signal, and an attB/attP-flanked PGK-FRT Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.
show more
close
|
| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
show more
close
|
| Breeding Considerations |
When maintaining a live colony, heterozygous mice may be bred with wildtype mice from the colony or to C57BL/6J inbred mice. The donating investigator has not attempted to breed homozygous mice together.
show more
close
|
| Breeding Strategy |
Wild-type x Heterozygote; Heterozygote x Wild-type
show more
close
|
For Research Use Only.
Related Products