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STOCK Shank3tm5.1Gfng/J

Cat. No.: CEMM-07250700

Common Name: Shank3fx (FLexed PDZ domain inverted)
Shank3fx is a cre-dependent FLEx switch allele containing an inverted Shank3 PDZ domain (exons 13-16) flanked by inward-facing tandem lox sites (lox2272:loxP). In the absence of Cre recombinase, this functions as a knockout allele and homozygotes exhibit autistic-like characteristics. Subsequent exposure to Cre recombinase that places the Shank3 PDZ domain into the proper orientation for expression results in restored expression of most major SHANK3 isoforms in those cells. These mice allow cre-inducible restoration of SHANK3 expression, and may be useful for studying autism spectrum disorder (ASD), Phelan-McDermid syndrome and schizophrenia.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Shank3tm5.1Gfng
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Allele Name
targeted mutation 5.1
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Allele Attributes
Conditional ready (e.g. floxed); Null/Knockout
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Gene Symbol
Shank3
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Gene Name
SH3 and multiple ankyrin repeat domains 3
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Chromosome
15
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MGI Accession ID
5775650
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Strain of Origin
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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Molecular Note
The targeting vector was designed with (from 5' to 3') an inward-facing lox2272:loxP site, an inverted Shank3 PDZ domain (exons 13-16), another inward-facing lox2272:loxP site and an FRT flanked neomycin resistance cassette all replacing the corresponding endogenous Shank3 sequences. Flp-mediated recombination removed the FRT flanked neo cassette.
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HUSBANDRY
Suggested Controls
B6129SF2/J
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Breeding Considerations
Homozygous mice are viable and fertile, with several autistic-like characteristics. When maintaining a live colony, heterozygous mice may be bred together, to wildtype littermates, or to C57BL/6J inbred mice.
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For Research Use Only.
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