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STOCK Mir137tm1Mtm/Mmjax
Cat. No.: CEMM-07250345
These microRNA 137 conditional mutant mice are designed to generate a null allele or a lacZ tagged null allele when combined with Flp or Cre recombinase expressing strains. This mutant mouse strain may be useful in studies of microRNA biology.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Mir137tm1Mtm
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Null/Knockout
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| Gene Symbol |
Mir137
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| Gene Name |
microRNA 137
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| Chromosome |
3
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| Expressed Genes |
lacZ, beta-galactosidase, E. coli
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| MGI Accession ID | show more close |
| Strain of Origin |
129P2/OlaHsd
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| Molecular Note |
A targeting vector was designed to insert an FRT site followed by a lacZ gene, a loxP site, a neomycin resistance gene cassette, a second FRT site and a second loxP site (FRT lacZ-loxP-Neo-FRT loxP) upstream of the microRNA stem loop and a third loxP site immediately downstream of the microRNA stem loop. This was intended to Create a knockout-first conditional-ready reporter knockout allele, but no beta-galactosidase activity could be detected in adult or embryonic tissues of heterozygous mice, nor could a functional conditional-ready allele be Created after flp-mediated deletion of the insert, suggesting that the inserted FRT and/or LoxP recombination sites interfere with the transcription of the allele.
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| HUSBANDRY | |
|---|---|
| Breeding Considerations |
While maintaining a live colony, these mice have been bred as heterozygotes, however, the Donating Investigator indicates that homozygotes are viable and fertile.
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For Research Use Only.
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