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STOCK Gt(ROSA)26Sortm8(ACTB-EGFP*, -tTA2)Luo/J
Cat. No.: CEMM-07250373
Common Name: R26G-tTA2
The R26G-tTA2 allele has a CMV enhancer/chicken beta-actin core promoter-driven "MADM G-tTA2" (GFPN-terminus-intron-tTA2ATG-less) inserted into the Gt(ROSA)26Sor locus on chromosome 6. These mutant mice are designed to combine the "new MADM-6" system with a Tet-Off binary expression system to create MADM-Tet (mosaic analysis with double markers combined with Tet-Off). This is a tool to generate genetic mosaics in which an individual organism contains somatic cells of different genotypes. This allows Cre or FLP recombinase-induced fluorescent labeling/tTA2-expression in daughter cells to ascertain lineal relationships and pleiotropic gene function in multicellular organisms. These mice are also a Tet-Off tool enabling a TRE transgene to be conditionally expressed in a subset of MADM-labeled cells.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm8(ACTB-EGFP*,-tTA2)Luo
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| Allele Name |
targeted mutation 8
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| Allele Attributes |
Reporter; Transactivator
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
GFP, Green Fluorescent Protein; tTA, tetracycline-controlled transactivator, E. coli
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| MGI Accession ID | show more close |
| Site of Expression |
Cre recombination results in EGFP and tetracycline-regulated transactivator sequence (tTA2) mosaic expression in Cre-expressing tissues When using the ?new MADM-6?system.
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| Strain of Origin |
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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| Molecular Note |
A targeting vector was Created with a CMV enhancer/chicken beta-actin core promoter (pCA) upstream of the MADM G-tTA2 cassette. The MADM G-tTA2 cassette used here (GFPN-terminus-intron-tTA2ATG-less) was designed with the N-terminal portion of a mutant enhanced green fluorescent protein (mut4EGFP first exon; nucleotides 1-274), a beta-globin intronic sequence (containing a FRT flanked region of two lox site variants [lox2272 and lox5171] and then a loxP-flanked neomycin resistance gene), an ATG-less tetracycline-regulated transactivator sequence (tTA2ATG-less), and an SV40 T-antigen poly(A) signal. This entire construct was subcloned into the pROSA26-PA targeting vector to generate the final targeting construct pROSA26-G-tTA2. The pROSA26-G-tTA2 construct was inserted into the Gt(ROSA)26Sor locus.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred together.
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For Research Use Only.
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