Online Inquiry
STOCK Gt(ROSA)26Sortm3(CAG-HRP)Ddg/J
Cat. No.: CEMM-07250998
Common Name: ROSA26DR-ER-HRP
ROSA26DR-ER-HRP mice conditionally express endoplasmic reticulum (ER)-targeted horseradish peroxidase (HRP) in a Cre and Flp dual recombinase (DR)-dependent manner. Concurrent Cre and Flp recombinase-mediated excision of loxP- and FRT-flanked Stop cassettes enables directed expression of horseradish peroxidase fused to an endoplasmic reticulum retention signal, useful in multiplexed electron microscopy labeling.The donating laboratory describes this strain as an ER-ERP strain: horseradish peroxidase fused to immunoglobulin kappa light chain leader sequence and KDEL endoplasmic reticulum retention signal.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm3(CAG-HRP)Ddg
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| Allele Name |
targeted mutation 3
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| MGI Accession ID | show more close |
| Strain of Origin |
129S4/SvJae
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| Molecular Note |
The targeting vector is Created by replacing the tdTomato cassette in Gt(ROSA)26Sortm65.1(CAG-tdTomato)Hze with DR-ER-HRP, a dual (Cre and Flp) recombinase (DR)-dependent, endoplasmic reticulum (ER)-targeted, horse radish peroxidase (HRP) reporter cassette. The following was introduced to intron 1 of the mouse Gt(ROSA)26Sor gene - CAG-FRT Stop-FRT loxP-Stop-loxP-ER ("KDEL" target peptide for endoplasmic reticulum retention)-HRP (horse radish peroxidase)-WPRE (Woodchuck Hepatitis Virus Posttranscriptional Regulatory Element)-bGHpA (bovine growth hormone polyadenylation signal)-attB-PGK-NeoR-PGKpA-attP.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
Wild-type from the colony
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| Breeding Considerations |
Heterozygotes and homozygotes are viable and fertile.
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| Breeding Strategy |
Heterozygote x Heterozygote
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For Research Use Only.
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