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B6.Cg-Gt(ROSA)26Sortm1(CAG-EGFP)Brsy/J
Cat. No.: CEMM-07250909
Common Name: MITO-Tag
MITO-Tag mice conditionally express, in a Cre recombinase-dependent manner, a hemagglutinin (HA)-tagged EGFP (codon-optimized) that localizes to mitochondrial outer membranes. These features enable immunocapture of mitochondria from specific cell types without the need for cell sorting techniques.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(CAG-EGFP)Brsy
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Epitope tag
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| MGI Accession ID | show more close |
| Strain of Origin |
B6(Cg)-Tyrc-2J
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| Molecular Note |
The targeting construct containing (from 5' t 3') CAG-loxP-neomycin (reversed)-pA-loxP-3xHA-EGFP-SYNJ2BP-lacZ-BGH-pA is targeted to intron 1 of the gene. The 3xHA-EGFP-SYNJ2BP components constitute the "MITO-Tag" cassette targeting the 3XHA epitope tag with GFP to the mitochondrial membrane. The construct includes only the transmembrane domain of the rat Synj2bp/OMP25 (AA109-145) gene. The small fragment of lacZ incorporated in the mutation is not expected to be functional.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
Hemizygotes and homozygotes retaining the floxed Stop cassette are viable and fertile.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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