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B6.Cg-Igs7tm161.1(tetO-EGFP, CAG-tTA2)Hze/J
Cat. No.: CEMM-07250874
Common Name: Ai161(TIT2L-GFP-ICR-tTA2)-D (or Ai161D)
Ai161(TIT2L-GFP-ICR-tTA2)-D (also called Ai161D) mice are a Dre and Cre-dependent, Tet-controllable fluorescent reporter line, created by targeted insertion at the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated). Exposure to Dre recombinase removes the rox-flanked STOP cassette and results in tTA2 expression. Subsequent exposure to Cre recombinase removes the loxP-flanked STOP cassette - allowing the tTA2 to induce EGFP expression. The EGFP expression may then be diminished by doxycycline. Ai161D mice allow intersectional genetic fate mapping of different cell subpopulations defined by the overlap of the two gene promoters driving the expression of each recombinase.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Igs7tm161.1(tetO-EGFP,CAG-tTA2)Hze
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| Allele Name |
targeted mutation 161.1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inducible; Transactivator
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| Gene Symbol |
Igs7
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| Gene Name |
intergenic site 7
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| Chromosome |
9
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| Expressed Genes |
GFP, Green Fluorescent Protein; tTA, tetracycline-controlled transactivator, E. coli
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| MGI Accession ID | show more close |
| Site of Expression |
When bred to mice expressing Dre and Cre recombinase in targeted tissues, the resulting offspring will have both STOP cassettes deleted in cells/tissues where the individual promoters driving Dre and Cre overlap - resulting in tTA2 expression and robust EGFP fluorescence in cells.
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| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The vector is designed with (from 5' to 3') an FRT3 site, two copies of chicken beta-globin HS4 insulator element (to reduce reporter gene expression in absence of transactivators), a Tet response element/promoter (see below), a loxP-flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-PGKpA), a synthetic enhanced green fluorescent protein sequence (EGFP), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a BGH polyA, two copies of chicken beta-globin HS4 insulator element, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from pCAGGS), a rox-flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-TKpA), a synthetic modified tetracycline-regulated transactivator gene (tTA2S>), a WPRE, a BGH polyA, an AttB site, a PGK-5'hygro cassette, an RNA splice donor and a FRT5 site. The TRE2 promoter used here is Tet-responsive P>hCMV*-1min CMVhCMV*-1< is silent in the absence of tTA or rtTA binding to tetO. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence and replaced it with the recombined AttB/AttP site (AttL).
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
Ai161D heterozygotes are viable and fertile with no reported gross physical or behavioral abnormalities. it has not been attempted to make this strain homozygous. When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice.
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| Breeding Strategy |
Wild-type x Heterozygote; Heterozygote x Wild-type
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For Research Use Only.
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