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STOCK Gt(ROSA)26Sortm1(CAG-COX4I1/APX1*)Ddg/J
Cat. No.: CEMM-07250928
Common Name: ROSA26DR-Matrix-dAPEX2
ROSA26DR-Matrix-dAPEX2 mice conditionally express mitochondrial matrix-targeted dimeric APEX2 (Matrix-dAPEX2, an enhanced soybean ascorbate peroxidase) in a cre and Flp dual recombinase-dependent manner. This multiplexed peroxidase-mediated electron microscopy (EM) labeling system facilitates the analysis of connectivity in the mammalian nervous system.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(CAG-COX4I1/APX1*)Ddg
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; No functional change; Epitope tag
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| MGI Accession ID | show more close |
| Strain of Origin |
129S4/SvJae
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| Molecular Note |
A CAG promoter, FRT flanked Stop cassette, loxP-flanked Stop cassette (in reverse transcriptional orientation), Matrix-dAPEX2, WPRE, bGHpA, attB, PGK-Neo, PGKpA, and attP were introduced to intron 1 via homologous recombination. APEX2 is an enhanced (K14D, W41F, E112K, A134P) soybean ascorbate peroxidase (APX1) that is less catalytically active than horseradish peroxidase (HRP), but is functional when expressed in cytosolic environments, unlike HRP. Dimeric APEX2 (dAPEX2) contains the reversion of K14 and E112 and is presumed to enhance heme affinity and thermal stability of the protein. Targeting of dAPEX2 to the mitochondrial matrix to Create Matrix-dAPEX2 was accomplished by a targeting peptide from human COX4I1 (MLATRVFSLVGKRAISTSVCVRAH) followed by a linker sequence (KDP) and V5 epitope tag. Matrix-dAPEX2 has the same sequence as mito-V5-APEX2 except for the replacement of APEX2 with dAPEX2. Flp-mediated recombination removed the FRT flank stop cassette.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
Wild-type from the colony
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| Breeding Considerations |
Heterozygotes and homozygotes are viable and fertile.
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| Breeding Strategy |
Heterozygote x Heterozygote
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For Research Use Only.
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