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B6.Cg-Gt(ROSA)26Sortm1(CAG-Pycard/mCitrine*, -CD2*)Dtg/J
Cat. No.: CEMM-07250823
Common Name: R26-CAG-LSL-ASC-citrine
The R26-CAG-LSL-ASC-citrine floxed allele has a CAG promoter and loxP-flanked STOP cassette upstream of the ASC-citrine fusion protein, all inserted into the Gt(ROSA)26Sor locus. Following removal of the floxed-STOP cassette, they express a fluorescent adaptor fusion protein (ASC-citrine) that retains the function of endogenous ASC - forming assembled inflammasome complexes (specks) upon exposure to inflammasome activator(s) both in vitro and in vivo. These R26-CAG-LSL-ASC-citrine mice are a Cre recombinase-dependent fluorescent reporter of inflammasome complex activation.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(CAG-Pycard/mCitrine*,-CD2*)Dtg
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
YFP, Yellow Fluorescent Protein, jellyfish
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| MGI Accession ID | show more close |
| Site of Expression |
Following Cre recombination the fluorescent adaptor fusion protein mASC-citrine is expressed in activated inflammasome complexes.
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| Strain of Origin |
B6.Cg-Thy1a
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| Molecular Note |
The targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, a loxP-flanked STOP cassette, the mASC-citrine-HA fusion protein (described below), an FRT site, an iIRES, a portion from the human CD2 antigen locus encoding a truncated signaling-deficient protein (hCD2*), a second FRT site and a BGH polyA signal. The targeting vector was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The mASC-citrine-HA fusion protein cDNA sequence encodes the mouse PYD and CARD domain containing coding region fused to a Citrine variant of yellow fluorescent protein, all followed by a hemagglutinin tag sequence. The Citrine used here is a yellow fluorescent protein variant with the K69A and A206K mutations (for improved brightness and monomeric stability) - encoding a ~5.7 pKa monomer with ~488nm excitation and ~529nm emmission spectra.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
Mice heterozygous or homozygous for the R26-CAG-LSL-ASC-citrine allele are viable and fertile with no reported gross physical or behavioral abnormalities. When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice. Alternatively, homozygous mice may be bred together.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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