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B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm1(tetO-cre)Haho/J
Cat. No.: CEMM-07250431
Common Name: R26-M2rtTA::TetOP-Cre
These double mutant R26-M2rtTA::TetOP-Cre mice utilize the rtTA/tetO (tet-on/TRE) system to allow highly-efficient, doxycycline-inducible Cre recombinase expression in hematopoietic stem cells. These mice harbor the R26M2rtTA targeted mutation (an optimized form of reverse tetracycline controlled transactivator [rtTA-M2] downstream of the Gt(ROSA)26Sor promoter) and the Col1a1TetOP-Cre targeted mutation (a tetracycline responsive element-driven Cre recombinase targeted to the collagen type I alpha 1 locus).
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(rtTA*M2)Jae
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Transactivator
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
rtTA, reverse tetracycline-controlled transactivator, E. coli
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| MGI Accession ID | show more close |
| Site of Expression |
Expresses an optimized rtTA protein (rtTA-M2). Inducible target gene expression is detected in liver, bone marrow, stomach, intestine, and skin, with lower levels in the heart, lungs, kidney, spleen, and thymus; no expression is detected in the brain and testes.
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| Strain of Origin |
(C57BL/6 x 129S4/SvJae)F1
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| Molecular Note |
An optimized form of reverse tetracycline controlled transactivator (rtTA-M2) was inserted downstream of the Gt(ROSA)26Sor promoter and was followed by a PGK-puro selection cassette. This mutant form of rtTA termed M2 has five amino acid substitutions in the tetR moiety of tTA: S12G, E19G, A56P, D148E and H179R. This mutated form of transactivator protein has inCreased doxycycline sensitivity. Mice have widespread expression of the rtTA-M2 protein.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
B6129SF2/J
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| Breeding Considerations |
When maintaining a live colony, mice that are homozygous for both mutations may be bred together.
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For Research Use Only.
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