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B6;129S-Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze/J
Cat. No.: CEMM-07250227
Common Name: Ai32; Ai32(RCL-ChR2(H134R)/EYFP)
Ai32 mice express an improved channelrhodopsin-2/EYFP fusion protein following exposure to Cre recombinase. These mice can be used in optogenetic studies for rapid in vivo activation of excitable cells by illumination with blue light (450-490 nm).
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm32(CAG-COP4*H134R/EYFP)Hze
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| Allele Name |
targeted mutation 32
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| Allele Attributes |
Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
COP4, Channelrhodopsin, Chlamydomonas
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| MGI Accession ID | show more close |
| Site of Expression |
Cre excision of the stop signal results in expression of an improved channelrhodopsin-2/ EYFP fusion protein (hChR2(H134R)- EYFP) in Cre-expressing tissues.
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| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The Rosa-CAG-LSL-ChR2(H134R)-EYFP-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), a ChR2(H134R)-EYFP fusion gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a BGH polyA signal, and an attB/attP-flanked PGK-FRT Neo-polyA cassette. To Create the ChR2(H134R)-EYFP fusion gene, a cDNA sequence encoding the first 315 amino acids of channelrhodopsin-2 (derived from the green alga Chlamydomonas reinhardtii) was modified with a gain-of-function H134R substitution (CAC to CGC) designed to cause larger stationary photocurrents. This ChR2(H134R) sequence was fused in-frame to the amino terminus of an enhanced yellow fluorescent protein sequence, resulting in the final ChR2(H134R)-EYFP fusion protein sequence. This entire construct was inserted between exons 1 and 2 of the locus.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
B6129SF2/J
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| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred together.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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